D-Luciferin, (CAS: 2591-17-5) also known as Firefly Luciferin, is a light-emitting compound, found in many firefly (Lampyridae) species. It is the substrate of luciferase (EC 1.13.12.7), which is responsible for the characteristic yellow light emission from many firefly species. As with all other luciferins, oxygen is required to elicit light; however, it has also been found that adenosine triphosphate (ATP) and magnesium are required for light emission. D-Luciferin is a substrate for firefly luciferase with a Km of approx 2 μM.
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